8IDQ

Crystal structure of reducing-end xylose-releasing exoxylanase in GH30 from Talaromyces cellulolyticus with xylose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 2
Unit cell lengths	138.249, 120.159, 122.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.350 - 1.700
R-factor	0.2007
Rwork	0.199
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.896
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	MOLREP (11.6)
Refinement software	PHENIX (11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.940	1.760
High resolution limit [Å]	1.700	1.700
Rpim	0.107	0.395
Number of reflections	222295	22012
<I/σ(I)>	9	2.4
Completeness [%]	99.2
Redundancy	4.9
CC(1/2)	0.977	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	22% PEG 6000, 0.1 M Tris-HCl, 0.2 M lithium chloride