8IDP

Crystal structure of reducing-end xylose-releasing exoxylanase in GH30 from Talaromyces cellulolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 2
Unit cell lengths	138.457, 120.072, 122.834
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.940 - 1.800
R-factor	0.1666
Rwork	0.165
R-free	0.19480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.894
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	MOLREP (11.6)
Refinement software	PHENIX (1.12)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.960	1.860
High resolution limit [Å]	1.800	1.800
Rpim	0.050	0.257
Number of reflections	188427	97250
<I/σ(I)>	9.3	2.7
Completeness [%]	97.9
Redundancy	5
CC(1/2)	0.994	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG 6000, 0.1 M Tris-HCl, 0.2 M lithium chloride