8IDO

Crystal structure of nanobody VHH-T148 with MERS-CoV RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	175.896, 47.190, 124.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.800 - 2.500
R-factor	0.2223
Rwork	0.220
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.800	2.589
High resolution limit [Å]	2.500	2.500
Number of reflections	36441	3443
<I/σ(I)>	14.45
Completeness [%]	99.0
Redundancy	5.8
CC(1/2)	0.987	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	4% v/v Tacsimate pH 6.0, 12% w/v PEG 3350