8IC6
exo-beta-D-arabinanase ExoMA2 from Microbacterium arabinogalactanolyticum in complex with Tris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.552, 97.511, 139.978 |
| Unit cell angles | 90.00, 100.55, 90.00 |
Refinement procedure
| Resolution | 48.800 - 1.750 |
| R-factor | 0.13941 |
| Rwork | 0.138 |
| R-free | 0.17386 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.881 |
| Data reduction software | XDS (Jan 10, 2022) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.760 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.046 | 0.366 |
| Rpim | 0.043 | 0.246 |
| Number of reflections | 176777 | 8703 |
| <I/σ(I)> | 14.8 | 2.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.874 | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.9 | 293.15 | 0.18 M MgCl2, 0.1 M Tris-HCl (pH 9.0), 14% PEG8000 |
