8IBO
Crystal structure of Wild-Type Mycobacterium tuberculosis ClpC1 N-terminal domain in complex with Lassomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2019-09-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.431, 58.468, 64.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.860 - 1.830 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.546 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.860 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.038 | 0.497 |
| Number of reflections | 16474 | 1650 |
| <I/σ(I)> | 30.4 | 4 |
| Completeness [%] | 100.0 | |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 1.4 M sodium acetate, 0.1 M sodium cacodylate (pH 6.5) |
