8IBI
Inactive mutant of CtPL-H210S/F214I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-14 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34138 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 65.316, 153.881, 52.996 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.320 - 2.140 |
| R-factor | 0.17166 |
| Rwork | 0.169 |
| R-free | 0.22073 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cwq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.610 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.320 | 2.170 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.116 | 0.368 |
| Number of reflections | 30315 | 1224 |
| <I/σ(I)> | 12.52 | 3.79 |
| Completeness [%] | 99.9 | |
| Redundancy | 9.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 20% PEG 2000 MME, 0.2 M ammonium citrate tribasic pH7.0, 0.1 M imidazole pH7.0 |
