8IB1

Structure of the LAH31 Fab bound to an influenza virus HA epitope peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	68.050, 86.510, 89.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.980 - 1.950
R-factor	0.187
Rwork	0.185
R-free	0.22520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wrp 5wl2
RMSD bond length	0.005
RMSD bond angle	0.763
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.980	2.060
High resolution limit [Å]	1.950	1.950
Number of reflections	73864	5812
<I/σ(I)>	10
Completeness [%]	98.7
Redundancy	6.2
CC(1/2)	0.994	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Sodium acetate, Ammonium acetate, PEG4000