8IAN
Crystal structure of CtPL-H210S/F214I mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-31 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34138 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 115.356, 115.356, 94.713 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.790 - 2.080 |
| R-factor | 0.20289 |
| Rwork | 0.200 |
| R-free | 0.25426 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cwq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.595 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.790 | 2.110 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.138 | 0.593 |
| Number of reflections | 44073 | 1813 |
| <I/σ(I)> | 9.86 | 2.04 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.49 | 6.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M potassium bromide; 33% w/v PEG 2000 MME |
