8IA6
Crystal structure of scFv antibody against Phospholipase A2 of Echis carinatus venom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.988 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 66.890, 53.880, 169.326 |
| Unit cell angles | 90.00, 94.43, 90.00 |
Refinement procedure
| Resolution | 29.230 - 2.750 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.614 |
| Data reduction software | iMOSFLM |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.350 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 15296 | 1580 |
| <I/σ(I)> | 4.7 | 2.1 |
| Completeness [%] | 96.1 | 99.8 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.925 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.05 M Citric acid, 0.05 M Bis-Tris propane pH 5.0, 16% w/v Polyethylene glycol 3350 |
