8IA1

plastidial glycerol-3-phosphate acyltransferases (GPAT) from the green alga Myrmecia incisa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-07
Detector	MAR CCD 165 mm
Wavelength(s)	1.0001
Spacegroup name	P 1
Unit cell lengths	51.093, 81.553, 88.489
Unit cell angles	110.85, 106.79, 99.93

Refinement procedure

Resolution	37.320 - 2.310
R-factor	0.1862
Rwork	0.183
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.922
Data reduction software	HKL-2000 (HKL2000_v723-Linux)
Data scaling software	HKL-2000 (HKL2000_v723-Linux)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.320	2.380
High resolution limit [Å]	2.310	2.310
Number of reflections	50682	2902
<I/σ(I)>	16.1	2.61
Completeness [%]	89.2
Redundancy	2.2
CC(1/2)	0.990	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M (NH4)2SO4, 0.1M Tris-HCl pH 8.5, 20% (w/v) PEG 3350