8I9I

Glutamyl-tRNA synthetase from Escherichia Coli bound to Glutamate and Zinc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2011-10-06
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5419
Spacegroup name	C 1 2 1
Unit cell lengths	211.510, 61.290, 101.900
Unit cell angles	90.00, 96.69, 90.00

Refinement procedure

Resolution	43.644 - 3.300
R-factor	0.2554
Rwork	0.253
R-free	0.29630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.415
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.650	3.410
High resolution limit [Å]	3.300	3.300
Number of reflections	19872	1968
<I/σ(I)>	8.78
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.980	0.294

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M MOPS HEPESNa pH 7.7, 0.02M l-glu, 14% PEG 8000, 22% ehylene glycol