8I94

Structure of flavone 4'-O-glucoside 7-O-glucosyltransferase from Nemophila menziesii, complex with luteolin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-05-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	55.975, 75.226, 119.743
Unit cell angles	90.00, 96.50, 90.00

Refinement procedure

Resolution	48.330 - 2.430
R-factor	0.2415
Rwork	0.237
R-free	0.30940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.020
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.330	2.520
High resolution limit [Å]	2.430	2.430
Rmeas	0.222
Number of reflections	35086	3377
<I/σ(I)>	7.4
Completeness [%]	94.3
Redundancy	3.7
CC(1/2)		0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG3350, Hepes, Lithium Sulfate