8I90
Structure of flavone 4'-O-glucoside 7-O-glucosyltransferase from Nemophila menziesii, complex with UDP-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-04-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.654, 74.928, 112.404 |
| Unit cell angles | 90.00, 92.94, 90.00 |
Refinement procedure
| Resolution | 48.820 - 2.100 |
| R-factor | 0.2318 |
| Rwork | 0.230 |
| R-free | 0.28320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.975 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.820 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.170 | |
| Number of reflections | 51488 | 5068 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 95.3 | |
| Redundancy | 2.7 | |
| CC(1/2) | 0.806 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG3350, Hepes, Lithium Sulfate |
