8I90

Structure of flavone 4'-O-glucoside 7-O-glucosyltransferase from Nemophila menziesii, complex with UDP-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-04-13
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	55.654, 74.928, 112.404
Unit cell angles	90.00, 92.94, 90.00

Refinement procedure

Resolution	48.820 - 2.100
R-factor	0.2318
Rwork	0.230
R-free	0.28320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.975
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.820	2.180
High resolution limit [Å]	2.100	2.100
Rmeas	0.170
Number of reflections	51488	5068
<I/σ(I)>	7.8
Completeness [%]	95.3
Redundancy	2.7
CC(1/2)		0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG3350, Hepes, Lithium Sulfate