8I8Y
A mutant of the C-terminal complex of proteins 4.1G and NuMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 93.171, 93.171, 55.562 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.720 - 2.900 |
| R-factor | 0.2836 |
| Rwork | 0.281 |
| R-free | 0.34030 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.604 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 5420 | 551 |
| <I/σ(I)> | 18.36 | |
| Completeness [%] | 93.7 | |
| Redundancy | 20.4 | |
| CC(1/2) | 0.998 | 0.897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 90% (0.2 M Trimethylamine N-Oxide dihydrate, 0.1 M Tris, pH 9.4, 20% w/v Polyethylene glycol monomethyl ether 2,000) and 10% 0.1 M TCEP hydrochloride |
