8I8Q
Crystal structure of horse spleen L-ferritin H114A mutant at -180deg Celsius.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 93.15 |
| Detector technology | PIXEL |
| Collection date | 2022-08-02 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 182.219, 182.219, 182.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.100 - 1.500 |
| Rwork | 0.159 |
| R-free | 0.17410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.732 |
| Data reduction software | CrysalisPro (v42) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.109 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.064 | 0.438 |
| Rmeas | 0.068 | 0.478 |
| Number of reflections | 41908 | 2024 |
| <I/σ(I)> | 25 | |
| Completeness [%] | 100.0 | |
| Redundancy | 8.6 | |
| CC(1/2) | 0.999 | 0.918 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | Ammonium sulfate (0.5-1.0M), Cadmium sulfate(12-20mM) |
