8I83

Crystal Structure of phosphinothricin dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	95.130, 126.667, 223.741
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.220 - 2.620
R-factor	0.2002
Rwork	0.198
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.220	2.714
High resolution limit [Å]	2.620	2.620
Number of reflections	81285	8009
<I/σ(I)>	13.67	2.25
Completeness [%]	99.1	98.71
Redundancy	7.9
CC(1/2)	0.995	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1M Bis Tris Propane, pH 6.5, 0.2 M Sodium malonate dibasicmonohydrate, 20% w/v PEG 3350, 10 % v/v Ethylene glycol