8I78

Meso-Diaminopimelate dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-25
Detector	Bruker PHOTON II
Wavelength(s)	1.54178
Spacegroup name	H 3 2
Unit cell lengths	211.910, 211.910, 245.760
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.520 - 2.640
R-factor	0.18491
Rwork	0.183
R-free	0.21409
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.435
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (V1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.480	2.710
High resolution limit [Å]	2.640	2.640
Number of reflections	58941	4572
<I/σ(I)>	14.9
Completeness [%]	100.0
Redundancy	5.13
CC(1/2)	0.995	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	2M ammonium sulfate, 0.1M Sodium acetate trihydrate, pH 4.5