8I5K
Crystal structure of chitin oligosaccharide binding protein from Vibrio cholera in complex with chitotriose.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.087, 51.683, 70.328 |
| Unit cell angles | 90.00, 98.83, 90.00 |
Refinement procedure
| Resolution | 19.307 - 1.219 |
| R-factor | 0.1698 |
| Rwork | 0.169 |
| R-free | 0.18740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gfb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.938 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.307 | 1.280 |
| High resolution limit [Å] | 1.219 | 1.220 |
| Number of reflections | 237875 | 18620 |
| <I/σ(I)> | 8.82 | |
| Completeness [%] | 84.8 | |
| Redundancy | 3.45 | |
| CC(1/2) | 0.996 | 0.507 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 293 | 0.2 M Magnesium chloride hexahydrate, 20% w/v Polyethylene glycol 3350 |
