8I5J
Crystal structure of chitin oligosaccharide binding protein from Vibrio cholera.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.120, 81.643, 87.273 |
Unit cell angles | 90.00, 104.15, 90.00 |
Refinement procedure
Resolution | 19.810 - 1.602 |
R-factor | 0.1632 |
Rwork | 0.162 |
R-free | 0.19120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gf8 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.969 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.810 | 1.690 |
High resolution limit [Å] | 1.602 | 1.602 |
Number of reflections | 261371 | 28401 |
<I/σ(I)> | 8.75 | |
Completeness [%] | 91.6 | |
Redundancy | 3.27 | |
CC(1/2) | 0.994 | 0.519 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dehydrate, Polyethylene glycol 4,000 |