8I56
CfbA S11T variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-02 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 41 |
| Unit cell lengths | 67.060, 67.060, 82.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.460 - 2.840 |
| R-factor | 0.17471 |
| Rwork | 0.171 |
| R-free | 0.25326 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.960 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.840 | 10.000 | 2.840 |
| Rmerge | 0.046 | 0.025 | 0.858 |
| Rmeas | 0.048 | 0.026 | 0.888 |
| Number of reflections | 8721 | 209 | 92 |
| <I/σ(I)> | 30.32 | ||
| Completeness [%] | 100.0 | ||
| Redundancy | 13.8 | ||
| CC(1/2) | 1.000 | 1.000 | 0.881 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 0.3M Na-malonate, 0.1M Tris-HCl, 8% (w/v) Gamma-PGA |
