8I4N

Crystal strcuture of 6-phosphogluconate dehydrogenase from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-12
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	63.897, 120.297, 152.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.990 - 2.410
R-factor	0.20288
Rwork	0.200
R-free	0.26708
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.615
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (7.1.000)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.972	0.066	0.301
Total number of observations	142308
Number of reflections	74209	4292	3361
<I/σ(I)>	9.3
Completeness [%]	84.5	97.2	75.7
Redundancy	1.9	2.3	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	PEG 8000, Tris, MgCl2