8I4K
Structure of Azami Red1.0, a red fluorescent protein engineered from Azami Green
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 83.130, 72.770, 213.843 |
| Unit cell angles | 90.00, 99.48, 90.00 |
Refinement procedure
| Resolution | 72.500 - 1.840 |
| R-factor | 0.1782 |
| Rwork | 0.176 |
| R-free | 0.21730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.156 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.500 | 1.870 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.103 | 0.397 |
| Number of reflections | 109173 | 5391 |
| <I/σ(I)> | 6.7 | 2.5 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.989 | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1M Tris-HCl pH7.0, 0.2 M Ca(OAc)2 30% PEG3350 |
