8I4J
Structure of wild-type Azami Green from Galaxea fascicularis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.790, 71.350, 196.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.050 - 1.620 |
| R-factor | 0.1456 |
| Rwork | 0.145 |
| R-free | 0.17050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.385 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.050 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.090 | 0.484 |
| Number of reflections | 116315 | 5661 |
| <I/σ(I)> | 11.9 | 3.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 5.9 | |
| CC(1/2) | 0.993 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl pH8.5, 0.2M MgCl2, 20% PEG8000 |
