8I3G
Crystal structure of Eaf3-Eaf7 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.032, 42.088, 80.789 |
| Unit cell angles | 90.00, 95.35, 90.00 |
Refinement procedure
| Resolution | 36.390 - 2.400 |
| R-factor | 0.2128 |
| Rwork | 0.208 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f5j |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.741 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.240 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.073 | 0.580 |
| Number of reflections | 18423 | 1907 |
| <I/σ(I)> | 16.5 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.5 | 6.3 |
| CC(1/2) | 0.999 | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M tris pH 8.5, 25% w/v PEG 4000, 0.025% v/v Dichloromethane |
