8I3E
Crystal structure of ELKS1 in complex with Piccolo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.379, 46.111, 84.359 |
| Unit cell angles | 90.00, 110.44, 90.00 |
Refinement procedure
| Resolution | 31.110 - 2.700 |
| R-factor | 0.2479 |
| Rwork | 0.245 |
| R-free | 0.29790 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.644 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.245 | 1.650 |
| Rmeas | 0.256 | 1.723 |
| Rpim | 0.074 | 0.489 |
| Number of reflections | 11119 | 538 |
| <I/σ(I)> | 16 | 1.08 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 12.7 | 12.3 |
| CC(1/2) | 1.000 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1 M Sodium Formate pH 7.0, 12 % (w/v) PEG 3350 |
