8I3E

Crystal structure of ELKS1 in complex with Piccolo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97852
Spacegroup name	C 1 2 1
Unit cell lengths	110.379, 46.111, 84.359
Unit cell angles	90.00, 110.44, 90.00

Refinement procedure

Resolution	31.110 - 2.700
R-factor	0.2479
Rwork	0.245
R-free	0.29790
Structure solution method	AB INITIO PHASING
RMSD bond length	0.013
RMSD bond angle	1.644
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	Arcimboldo
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.245	1.650
Rmeas	0.256	1.723
Rpim	0.074	0.489
Number of reflections	11119	538
<I/σ(I)>	16	1.08
Completeness [%]	100.0	99.8
Redundancy	12.7	12.3
CC(1/2)	1.000	0.614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	0.1 M Sodium Formate pH 7.0, 12 % (w/v) PEG 3350