8I33

Coil 1a of lamin A (residue 25-65)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	22.933, 33.703, 49.229
Unit cell angles	97.28, 91.20, 94.63

Refinement procedure

Resolution	33.310 - 1.620
R-factor	0.1982
Rwork	0.197
R-free	0.22490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.709
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.650
High resolution limit [Å]	1.620	1.620
Rpim		0.108
Number of reflections	136711	18503
<I/σ(I)>	24.29	6.74
Completeness [%]	96.6	91.8
Redundancy	3.6	3.1
CC(1/2)	0.993	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	NaCl, PEG 6000, sodium thiocyanate