8I2F
Crystal structure of Bacillus subtilis LytE catalytic domain in complex with IseA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 11C |
| Synchrotron site | PAL/PLS |
| Beamline | 11C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.830, 74.640, 153.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.540 - 2.030 |
| R-factor | 0.1751 |
| Rwork | 0.173 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.796 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.540 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.088 | 0.539 |
| Rpim | 0.050 | 0.305 |
| Number of reflections | 45047 | 3272 |
| <I/σ(I)> | 9.1 | 2.2 |
| Completeness [%] | 98.3 | 98.5 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5 | 293 | 20% (w/v) PEG6000, 100 mM Citric acid/Sodium hydroxide pH 5.0 |
