8I2D
Crystal structure of Bacillus subtilis LytE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 55.250, 55.250, 68.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.900 - 1.310 |
| R-factor | 0.1745 |
| Rwork | 0.174 |
| R-free | 0.18370 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.754 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.070 | 1.330 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmerge | 0.082 | 0.446 |
| Rpim | 0.025 | 0.136 |
| Number of reflections | 26096 | 1260 |
| <I/σ(I)> | 20.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.5 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 20% (w/v) PEG3350, 200 mM Sodium thiocyanate |
