8I28

Structure of Phosphoserine Aminotransferase from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	132.278, 132.278, 141.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.330 - 2.800
R-factor	0.2309
Rwork	0.229
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6czx
RMSD bond length	0.010
RMSD bond angle	1.094
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	35761	3519
<I/σ(I)>	18.75
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.999	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2.4 M ammonium sulfate and 0.1 M citric acid (pH 3.5)