8I19
Crystal structure of human MTH1(G2K mutant) in complex with 8-oxo-dGTP at pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-23 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.498, 47.993, 123.489 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.630 - 1.480 |
| R-factor | 0.1525 |
| Rwork | 0.150 |
| R-free | 0.19010 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5ghi |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.108 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.630 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.080 | 0.268 |
| Number of reflections | 45875 | 2237 |
| <I/σ(I)> | 39.8 | |
| Completeness [%] | 99.4 | |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium citrate, tris, NaCl |
