8I0D

Sb3GT1 375S/Q377H mutant complex with UDP-Glc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	47.470, 74.520, 128.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.590 - 1.430
R-factor	0.14785
Rwork	0.146
R-free	0.18433
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.949
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.470	1.450
High resolution limit [Å]	1.430	1.430
Number of reflections	85370	4352
<I/σ(I)>	24.8
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)	1.000	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.05 M Citric acid, 0.05 M BIS-TRIS propane / pH 5.0), 16% w/v Polyethylene glycol 3,350.