8I0A
Structure of alpha-L-Arabinofuranosidase from Trametes hirsuta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 134.935, 134.935, 175.561 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.440 - 2.010 |
| R-factor | 0.1901 |
| Rwork | 0.190 |
| R-free | 0.19410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.206 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.440 | 2.071 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.224 | 2.778 |
| Number of reflections | 64082 | 6302 |
| <I/σ(I)> | 18.56 | |
| Completeness [%] | 99.9 | |
| Redundancy | 24.3 | 24.8 |
| CC(1/2) | 0.999 | 0.803 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 10000,potassium sodium tartrate tetrahydrate,Bis-TRIS |
