8I04

Crystal structure of serine acetyltransferase from Salmonella typhimurium complexed with serine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.90000
Spacegroup name	P 32 2 1
Unit cell lengths	122.157, 122.157, 128.184
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.990 - 2.300
R-factor	0.17392
Rwork	0.172
R-free	0.21019
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7e3y
RMSD bond length	0.010
RMSD bond angle	1.445
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.300	2.300
Rmerge	0.096	0.763
Rpim	0.103
Number of reflections	47207	7555
<I/σ(I)>	14.4	2.7
Completeness [%]	99.8	99
Redundancy	15.6
CC(1/2)	0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100 MM HEPES, 100 MM SODIUM CHLORIDE, 6% MPD