8HZV
The crystal structure of a Radical SAM Enzyme DesII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.186, 140.790, 116.021 |
Unit cell angles | 90.00, 96.28, 90.00 |
Refinement procedure
Resolution | 49.193 - 2.330 |
R-factor | 0.205792121245 |
Rwork | 0.204 |
R-free | 0.23478 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.575 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.200 | 2.370 |
High resolution limit [Å] | 2.330 | 2.330 |
Number of reflections | 94112 | 4668 |
<I/σ(I)> | 16.8 | |
Completeness [%] | 99.9 | |
Redundancy | 6.8 | |
CC(1/2) | 0.998 | 0.686 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 8.5, 32% PEG 4000, 0.2 M Lithium sulfate |