8HZL
A new fluorescent RNA aptamer_III bound with N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.620, 53.931, 81.826 |
Unit cell angles | 90.00, 105.87, 90.00 |
Refinement procedure
Resolution | 26.240 - 2.600 |
R-factor | 0.1842 |
Rwork | 0.181 |
R-free | 0.24370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8hze |
RMSD bond length | 0.005 |
RMSD bond angle | 1.062 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.112 | 0.047 | 0.854 |
Rmeas | 0.135 | 0.057 | 1.021 |
Rpim | 0.074 | 0.032 | 0.555 |
Total number of observations | 53549 | ||
Number of reflections | 16812 | 1733 | 1695 |
<I/σ(I)> | 4.9 | ||
Completeness [%] | 99.8 | 98.9 | 100 |
Redundancy | 3.2 | 3.1 | 3.3 |
CC(1/2) | 0.983 | 0.997 | 0.518 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M MgCl2, 20% PEG3350 |