8HZL

A new fluorescent RNA aptamer_III bound with N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	64.620, 53.931, 81.826
Unit cell angles	90.00, 105.87, 90.00

Refinement procedure

Resolution	26.240 - 2.600
R-factor	0.1842
Rwork	0.181
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8hze
RMSD bond length	0.005
RMSD bond angle	1.062
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.112	0.047	0.854
Rmeas	0.135	0.057	1.021
Rpim	0.074	0.032	0.555
Total number of observations	53549
Number of reflections	16812	1733	1695
<I/σ(I)>	4.9
Completeness [%]	99.8	98.9	100
Redundancy	3.2	3.1	3.3
CC(1/2)	0.983	0.997	0.518

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2 M MgCl2, 20% PEG3350