8HZ5
The homodimer of a biotin carboxylase isoform from chloroflexus aurantiacus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 54.790, 154.092, 206.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.100 - 3.000 |
| Rwork | 0.245 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-3000 (723) |
| Data scaling software | HKL-3000 (723) |
| Phasing software | PHASER (1.19.2-4158) |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.060 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 17211 | 1678 |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 95.4 | |
| Redundancy | 11.5 | |
| CC(1/2) | 0.884 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289.15 | 28% (v/v) isopropyl alcohol, 0.1M Bis-Tris pH 6.5, 4% (v/v) PEG200 |
