8HZ4
The tetrameric structure of biotin carboxylase from Chloroflexus aurantiacus in complex with bicarbonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 128.528, 126.224, 132.192 |
| Unit cell angles | 90.00, 106.44, 90.00 |
Refinement procedure
| Resolution | 28.800 - 3.200 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.687 |
| Data reduction software | HKL-3000 (723) |
| Data scaling software | HKL-3000 (723) |
| Phasing software | PHASER (1.19.2-4158) |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.800 | 3.314 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 66529 | 6647 |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 99.2 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.895 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289.15 | 0.1 M Bicine pH 9.0, 2% (v/v) 1,4-Dioxane, 10% (w/v) PEG20,000, 5% (w/v) PEG550 |
