8HZ2

Tumor Necrosis Factor Receptor Associated Factor 6 (TRAF6) N-terminal Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU PhotonJet-R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-10
Detector	RIGAKU HyPix-3000
Wavelength(s)	1.54
Spacegroup name	P 1
Unit cell lengths	45.885, 51.705, 54.300
Unit cell angles	91.04, 112.15, 108.43

Refinement procedure

Resolution	5.998 - 2.600
R-factor	0.3541
Rwork	0.348
R-free	0.40860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hcs
RMSD bond length	0.002
RMSD bond angle	0.538
Data reduction software	CrysalisPro (1.171.42.35a)
Data scaling software	CrysalisPro (1.171.42.35a)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	5.998	2.685
High resolution limit [Å]	2.600	2.600
Number of reflections	12134	1157
<I/σ(I)>	3.12
Completeness [%]	90.5
Redundancy	1.5
CC(1/2)	0.920	0.380

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	1.2 M Sodium Citrate Tribasic Dihydrate and 0.1 M TRIS 8.5