8HYG
Crystal structure of protease-associated domain of Arabidopsis vacuolar sorting receptor 1 at pH 4.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-09-05 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.999840 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.987, 60.318, 38.466 |
| Unit cell angles | 90.00, 103.36, 90.00 |
Refinement procedure
| Resolution | 31.800 - 1.600 |
| R-factor | 0.1719 |
| Rwork | 0.169 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tjv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.695 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.420 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 19332 | 954 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 6.4 | 4.2 |
| CC(1/2) | 0.997 | 0.933 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% w/v Polyethylene glycol 4,000 |
