8HW6
Crystal structure of Heterodera glycines chitinase 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.802, 69.347, 50.564 |
| Unit cell angles | 90.00, 105.27, 90.00 |
Refinement procedure
| Resolution | 29.488 - 1.923 |
| R-factor | 0.1581 |
| Rwork | 0.155 |
| R-free | 0.18520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.824 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.992 |
| High resolution limit [Å] | 1.923 | 1.923 |
| Rmerge | 0.121 | 0.421 |
| Rmeas | 0.135 | 0.473 |
| Number of reflections | 23016 | 2030 |
| <I/σ(I)> | 16.23 | 4.03 |
| Completeness [%] | 97.0 | 86 |
| Redundancy | 4.8 | 4.7 |
| CC(1/2) | 0.989 | 0.851 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M ammonium acetate, 0.1 M Bis-tris, pH 5.5, and 20% PEG 10,000 |
