8HW5
Crystal structure of African swine fever virus CP312R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.993, 79.799, 47.545 |
| Unit cell angles | 90.00, 118.77, 90.00 |
Refinement procedure
| Resolution | 24.070 - 2.300 |
| R-factor | 0.1914 |
| Rwork | 0.186 |
| R-free | 0.23780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.587 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
| Rmerge | 0.992 | 0.047 | 0.250 |
| Total number of observations | 85579 | ||
| Number of reflections | 13790 | 1313 | 1329 |
| <I/σ(I)> | 12.8 | ||
| Completeness [%] | 98.8 | 97.1 | 98.2 |
| Redundancy | 3.2 | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | magnesium chloride BIS-TRIS PEG 3350 |
