8HVD

Crystal structure of lacto-N-biosidase StrLNBase from Streptomyces sp. strain 142, galacto-N-biose complex 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-28
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	1.000000
Spacegroup name	P 1 21 1
Unit cell lengths	52.779, 60.825, 88.232
Unit cell angles	90.00, 98.19, 90.00

Refinement procedure

Resolution	47.940 - 1.410
R-factor	0.16594
Rwork	0.165
R-free	0.19273
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.724
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.990	1.430
High resolution limit [Å]	1.410	1.410
Rmerge	0.051	0.482
Rmeas	0.061	0.583
Rpim	0.033	0.324
Number of reflections	107633	16650
<I/σ(I)>	12.4	2.1
Completeness [%]	99.9	100
Redundancy	3.4	3.1
CC(1/2)	0.995	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.25 M Ammonium fluoride, 12.5%(w/v) PEG 3350