8HUA
Serial synchrotron crystallography structure of ba3-type cytochrome c oxidase from Thermus thermophilus using a goniometer compatible flow-cell
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2019-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.060, 100.170, 96.620 |
| Unit cell angles | 90.00, 126.76, 90.00 |
Refinement procedure
| Resolution | 25.802 - 2.120 |
| Rwork | 0.152 |
| R-free | 0.18440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.720 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.802 | 2.200 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Number of reflections | 65010 | 6268 |
| <I/σ(I)> | 6.8 | |
| Completeness [%] | 100.0 | |
| Redundancy | 123.6 | |
| CC(1/2) | 0.990 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.3 | 298 | 1.4 M NaCl, 100 mM MES pH 5.3 with 36 % to 39 % (v/v) PEG 400 |
