8HU4
Limosilactobacillus reuteri N1 GtfB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 150 |
| Detector technology | PIXEL |
| Collection date | 2022-07-26 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 204.420, 100.730, 106.890 |
| Unit cell angles | 90.00, 108.14, 90.00 |
Refinement procedure
| Resolution | 47.210 - 2.760 |
| R-factor | 0.24849 |
| Rwork | 0.248 |
| R-free | 0.26609 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.210 | 2.840 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.103 | 0.348 |
| Number of reflections | 52935 | 4564 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.7 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 4% v/v MPD, 0.1 M Citric acid pH 3.5, 20% PEG 1500 |
