8HTX
Crystal structure of BANP in complex with methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 42.352, 92.645, 127.022 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.760 - 2.800 |
| R-factor | 0.2344 |
| Rwork | 0.232 |
| R-free | 0.27230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | The solved BANP structure in this study |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.636 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.510 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 12915 | 1818 |
| <I/σ(I)> | 12.6 | 3.8 |
| Completeness [%] | 99.7 | |
| Redundancy | 10.8 | |
| CC(1/2) | 0.995 | 0.979 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium fluoride, 0.1 M BTP-tris (pH 6.5), 20 % PEG3350 (w/v) |
