8HTD
Crystal structure of an effector from Chromobacterium violaceum in complex with ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97890 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.520, 74.644, 50.358 |
Unit cell angles | 90.00, 99.23, 90.00 |
Refinement procedure
Resolution | 41.373 - 1.848 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.22530 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3228) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.880 |
High resolution limit [Å] | 1.848 | 5.020 | 1.850 |
Rmerge | 0.083 | 0.063 | 0.261 |
Rmeas | 0.098 | 0.073 | 0.306 |
Rpim | 0.050 | 0.037 | 0.158 |
Number of reflections | 22527 | 1162 | 1167 |
<I/σ(I)> | 9.1 | ||
Completeness [%] | 93.3 | 93.1 | 97.4 |
Redundancy | 3.7 | 3.7 | 3.6 |
CC(1/2) | 0.993 | 0.901 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 100 mM sodium cacodylate, pH 6.5, 200 mM sodium acetate, 28% to 30% PEG 8000 |