8HT0

Improved thermostability of a glucose-tolerant glycosidase based on its X-ray crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97915
Spacegroup name	I 4 2 2
Unit cell lengths	166.300, 166.300, 122.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.197 - 2.000
R-factor	0.1971
Rwork	0.196
R-free	0.22580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.813
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.160	2.010
High resolution limit [Å]	1.960	1.960
Number of reflections	61362	4500
<I/σ(I)>	21.3
Completeness [%]	100.0
Redundancy	26.4
CC(1/2)	1.000	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	0.1M Citric acid pH 4.0,1.4M Ammonium sulfate pH 5.0