8HS7
Crystal structure of Vesicle-associated membrane protein-associated protein SCS2 from yeast
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978520 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.689, 68.689, 56.374 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.920 - 2.000 |
| R-factor | 0.2505 |
| Rwork | 0.249 |
| R-free | 0.26060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.627 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4778) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | 0.643 |
| Rpim | 0.012 | 0.159 |
| Number of reflections | 10680 | 1312 |
| <I/σ(I)> | 37.12 | 4.49 |
| Completeness [%] | 99.8 | |
| Redundancy | 19 | |
| CC(1/2) | 1.000 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 25% PEG 3350, 0.1 mM tris-bis (pH 5.5). 0.1 M EDTA |
