8HRF
Catalytic domain of Vibrio parahaemolyticus chitinase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2017-05-24 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.804, 159.996, 75.489 |
| Unit cell angles | 90.00, 92.68, 90.00 |
Refinement procedure
| Resolution | 28.700 - 2.500 |
| R-factor | 0.1649 |
| Rwork | 0.161 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.898 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.700 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.149 | 0.330 |
| Number of reflections | 39898 | 3994 |
| <I/σ(I)> | 13.3 | 5.63 |
| Completeness [%] | 98.8 | 97.42 |
| Redundancy | 7.2 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 10% PEG 8000, 100 mM CaAc2, 100 mM Na-cacodylate |
