8HR0
The complex structure of COPII coat with HCoV-OC43 DD sorting motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.580, 96.203, 130.465 |
| Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
| Resolution | 49.023 - 3.340 |
| R-factor | 0.2286 |
| Rwork | 0.223 |
| R-free | 0.29910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.344 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16.3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.450 |
| High resolution limit [Å] | 3.340 | 3.340 |
| Rmerge | 0.126 | |
| Number of reflections | 26475 | 2430 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 98.2 | |
| Redundancy | 2.5 | |
| CC(1/2) | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 281.15 | 50 mM Tris-HCl, pH 7.9, 0.2 M NaAc and 16% PEG4000 |
